Fuengirola and Mijas are no longer under alert for West Nile virus.
It comes after the Junta de Andalucia lifted the special measures that had been in place in both Costa del Sol municipalities.
The regional government confirmed the change on Monday in its latest update on the mosquito-borne virus, meaning the extraordinary surveillance and mosquito-control measures associated with the alerts can now be stood down.
Crucially, neither Fuengirola nor Mijas has recorded a confirmed human case of West Nile virus during the current outbreak.
However, the threat has not disappeared across Andalucia, with 26 municipalities still under alert and the number of confirmed human infections this season reaching 52.
The latest case is a person in Huevar del Aljarafe, Sevilla province, who has developed a mild form of the illness.
Being declared an alert area triggers enhanced monitoring for West Nile virus across several fronts.
This includes increased entomological, animal and human surveillance, as well as additional public information campaigns involving pharmacies and nursing services.
Local authorities must also intensify efforts to identify and control mosquito populations capable of transmitting the virus.
Measures extend beyond built-up areas to potential mosquito breeding sites and adult mosquito refuges located within 1.5km of population centres.
Those enhanced measures have now been lifted in Fuengirola and Mijas, as well as Gelves in Sevilla province.
Huevar del Aljarafe, however, has been added to the alert list following its newly confirmed human case and will remain under alert until September 21.
52 cases and one death across Andalucia
A total of 52 people have now contracted West Nile virus in Andalucia this season.
Of those, 36 developed mild illness, while 16 suffered the more serious neuroinvasive form of the disease.
Nineteen patients have required hospital treatment over the summer, with four currently remaining in hospital, two fewer than last Friday.
One person has died, with the fatality recorded on July 27.
Cases have overwhelmingly been concentrated in Sevilla province.
Eight have been recorded in Dos Hermanas, six in Coria del Rio, five in La Puebla del Rio and four each in Almensilla and Aznalcazar.
Villamanrique de la Condesa has recorded the largest number, with 11 confirmed infections.
Other cases have been detected in Bollullos de la Mitacion, Los Palacios, Palomares del Rio, Sevilla city, San Juan de Aznalfarache, Olivares and Huevar del Aljarafe.
The only confirmed case outside Sevilla province has been in Hinojos, Huelva.
The Junta has also expanded surveillance of people suffering milder symptoms this season, with 515 people undergoing laboratory testing to determine whether they had contracted the virus.
